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Immunology and Infection

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JoVE Journal Immunology and Infection
Preparation of Electrocompetent Cells to Achieve Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain
 

Preparation of Electrocompetent Cells to Achieve Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain

Single-Copy Gene Expression for Characterizing Multidrug Efflux Systems

Article DOI: 10.3791/201441-v • 01:17 min • January 5th, 2024
January 5th, 2024 •
1, 1
1Department of Microbiology, University of Manitoba

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Keywords: Acinetobacter Baumannii Multidrug Resistance Efflux Pumps RND-type Efflux Single-copy Gene Expression Mini-Tn7 System Efflux-deficient Bacterial Strain Characterization Of Efflux Systems Antibiotic Resistance Mechanisms Genetic Manipulation Tools
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