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Sectioning, Staining and TEM Imaging of Embedded Exosomes: A Protocol To Visualize the Structural Features of Exosomes Using TEM
Transcript
Using an ultra-microtome, prepare sections with a thickness of 60 nanometers. Place the sections on a nickel grid and double stain some of them with 2% uranyl acetate for 20 minutes, followed by lead citrate for 10 minutes. Next, place the stained section into a transmission electron microscope and view the sample using 80 kV and follow automatic settings for the exposure time. With an exosome image in view, acquire and save the image using the microscope's software.
