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The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning
DOI:

05:00 min

September 05, 2018

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Chapters

  • 00:04Title
  • 00:27Assemble the Equipment and Connect the Microtome
  • 01:51Paraffin Sectioning
  • 03:24Results: Analysis of the Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning
  • 04:32Conclusion

Summary

Automatic Translation

Here, we present a protocol to improve paraffin sectioning. This method combines cutting and floating using a simple thermostatic chamber to avoid the transfer process required by the conventional method. As a result, the efficiency and the number of intact paraffin sections were greatly improved.

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