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髓母细胞瘤干细胞的分离,浓缩,和维修
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Isolation, Enrichment, and Maintenance of Medulloblastoma Stem Cells
DOI:

06:32 min

September 01, 2010

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Chapters

  • 00:00Title
  • 00:59Introduction
  • 01:20Micro-dissection of Tumor-bearing Cerebellum and Dissociation of Tumor Tissue
  • 03:34Enrichment, Maintenance, and Expansion of Medulloblastoma Tumor Cells by Serial Passages
  • 04:26Immunofluorescent Staining of Cultured Medulloblastoma Cells
  • 04:53Morphology, Lineage Differentiation, and Expression of Stem Cells Markers
  • 06:17Conclusion

Summary

Automatic Translation

这个协议描述和异位刺猬通路活性的突变小鼠的髓母细胞瘤肿瘤干细胞的分离,浓缩,维护。

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