Performing Injection into Orbital Lobe of Superior Lacrimal Gland: A Technique for Inducing Dry Eye Disease in Rabbit Model

Apply medial pressure to the globe to cause the orbital superior lacrimal gland to protrude from the posterior incisure. Using closed curved forceps, indent the area until the bony opening in the skull is felt, and apply further modest pressure with forceps to leave an indentation in the skin to serve as the landmark for the needle placement.

Insert a tuberculin syringe equipped with a 27-gauge needle perpendicular to the skin over the indentation mark about a 1/4 inch into the incision, and redirect the needle posteriorly and externally toward the lateral canthus, aiming for the midpoint between the injection site and the bony orbital rim. Once the hub of the needle is reached, slowly inject 0.2 milliliters of a 1,000 microgram solution of conA.