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Fabrication of Biologically Derived Injectable Materials for Myocardial Tissue Engineering

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Fabrication of Biologically Derived Injectable Materials for Myocardial Tissue Engineering

Fabrication of Biologically Derived Injectable Materials for Myocardial Tissue Engineering

DOI:

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11:32 min

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December 20, 2010

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Sonya Seif-Naraghi, Jennifer Singelyn, Jessica DeQuach, Pamela Schup-Magoffin, Karen Christman

¹University of California, San Diego

Chapters

00:05Title
00:59Introduction
01:34Pre-processing Tissue Preparation
02:52Preparation of Solubilized Extracellular Matrix
04:56Myocardial Injection
09:57Representative Immunohistochemistry
11:02Conclusion

Summary

Automatic Translation

Methods for preparing an injectable matrix gel from decellularized tissue and injecting it into rat myocardium in vivo are described.

Tags

Biologically Derived Injectable Materials Extracellular Matrix Gel Lyophilization Enzymatic Digestion Physiological PH ECM Powder Pepsin Digestion Injectable Matrix Characterization Assays Native ECM Components Proteins Peptides Glycosaminoglycans Intramural Injection Left Ventricular Free Wall Small Animal Model Biotin-labeling Horse Radish Peroxidase-conjugated Neutravidin

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