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3幼虫节段性神经的可视化 RD龄果蝇幼虫的筹备工作
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JoVE Journal Neuroscience
Visualization of Larval Segmental Nerves in 3rd Instar Drosophila Larval Preparations
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3幼虫节段性神经的可视化 RD龄果蝇幼虫的筹备工作

DOI:
10.3791/2128-v

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07:18 min

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September 29, 2010

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, , ,
1Department of Biological Sciences,SUNY-University at Buffalo

Chapters

  • 00:00Title
  • 00:49Introduction
  • 01:10Dissection of 3rd Instar Larvae
  • 03:20Fixation and Antibody Staining of the Dissected Larva
  • 05:06Mounting and Visualization of the Dissected Larva
  • 06:17Representative Images of Drosophila Larvae Segmental Nerves
  • 06:49Conclusion

Summary

Automatic Translation

Automatic Translation

果蝇幼虫提供了一个理想的模型系统调查内幼虫节段性神经轴突运输的机制。使用此过程中， 第三龄幼虫携带各种突变可以比野生型幼虫。

Tags

Larval Segmental Nerves3rd Instar Drosophila LarvaeNervous System DevelopmentAxonal TransportSynaptic Vesicle ProteinsVisualizedLarval PreparationsDrosophila MelanogasterGeneticsFully Sequenced GenomeAxonsCell BodiesNerve TerminalsSynaptic Vesicle AntibodiesWild Type Larvae

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