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微机械动态细胞培养平台
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Microfabricated Platforms for Mechanically Dynamic Cell Culture
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Click here for the English version.
微机械动态细胞培养平台
DOI:
10.3791/2224-v
•
15:21 min
•
December 26, 2010
•
Christopher Moraes
2
,
Yu Sun
2
,
Craig A. Simmons
2,3
1
Department of Mechanical and Industrial Engineering
,
University of Toronto
,
2
Institute of Biomaterials and Biomedical Engineering
,
University of Toronto
,
3
Faculty of Dentistry
,
University of Toronto
Chapters
00:05
Title
01:05
Introduction
01:31
Sandwich Mold Fabrication
03:27
Constructing the Multilayer Device
06:45
Microactuator Modification for Active 2D Substrates
10:01
Device Modifications for Active 3D Hydrogels
12:11
In Situ Polymerization
13:51
Representative Results for Device Fabrication, Characterization, and Operation
14:57
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这个协议中,我们展示的微驱动器阵列的垂直流离失所该技术是基于职位的制造,以及如何可以修改此基础技术,进行二维和三维培养高吞吐量的机械动态细胞培养范例。
Tags
Microfabricated Platforms
Mechanically Dynamic Cell Culture
Mechanobiological Stimuli
Drug Discovery
Cell Biology Studies
Bioreactor Technologies
Mechanical Stimuli
High-throughput Format
Microactuator Array
Vertically Displaced Posts
Two-dimensional Culture Paradigms
Three-dimensional Culture Paradigms
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