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Chapters
- 00:05Title
- 01:49Yeast Colony Isolation and Fixation
- 03:03Washes, Osmium Treatment, and Dehydration
- 04:20Spurr’s Reagent Preparation and Infiltration
- 06:10Microbial Colony Structure of Embedded and Sectioned Yeast
- 07:55Conclusion
A method for embedding yeast colonies allowing sectioning for light and electron microscopy. This protocol allows determination of the distribution of sporulated cells and pseudohyphal cells within colonies providing a new tool toward understanding the organization of cell types within a fungal community.
Tags
Yeast Colony Embedding MethodPatterningCell TypesMetazoan DevelopmentMicroorganismsColoniesBiofilmsYeast S. CerevisiaeSporulated CellsPseudohyphal CellsFunctional ImportanceMolecular MechanismsInvestigating PatternsFungal ColoniesCell AttachmentExtracellular MatrixEmbedding And Sectioning MethodInterior PatternsThick SectionsLight MicroscopyTransmission Electron MicroscopyAsci CellsPseudohyphal CellsOvoid Yeast Cells
