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单克隆抗体的反对消极行政 H。 capsulatum和其他真菌病原体
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Immunology and Infection
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JoVE Journal Immunology and Infection
Passive Administration of Monoclonal Antibodies Against H. capsulatum and Others Fungal Pathogens
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单克隆抗体的反对消极行政 H。 capsulatum和其他真菌病原体

DOI:
10.3791/2532-v

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09:57 min

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February 14, 2011

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, ,
1Department of Microbiology and Immunology,Albert Einstein College of Medicine

Chapters

  • 00:05Title
  • 02:01Growth of H. capsulatum
  • 03:50Hybridoma Growth and Purification of Monoclonal Antibodies
  • 04:37Mouse Anesthesia and Intranasal Infections
  • 06:22Survival Studies
  • 07:17CFU Studies
  • 08:27Monoclonal Antibody Protection Against H. capsulatum is Dependent on Antibody Specificity and Isotype
  • 09:18Conclusion

Summary

Automatic Translation

Automatic Translation

C57BL / 6小鼠已被用于研究HC发病机制，并提供最好的模式。我们正在探索这种真菌对体液免疫的潜在利益，并产生了几个单克隆抗体[组蛋白H2B和热休克蛋白60kDa]，我们为他们的腹腔给药后的保护效果测试。

Tags

Passive AdministrationMonoclonal AntibodiesH. CapsulatumFungal PathogensHistoplasmosisAntibody TherapyVirulence FactorsMAbs GenerationSolid Phase ELISA SystemSplenocytes FusionC57BL/6 MiceInfection AssessmentMortality As An EndpointColony Forming Units (CFU)Survival StudiesIntervention EfficacyVaccine EfficacyVirulence Testing

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