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High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors
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Medicine
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JoVE Journal Medicine
High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors

High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors

DOI:
10.3791/2538-v

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19:02 min

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March 22, 2011

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, , , , , , , ,
1Department of Pediatrics, Division of Cellular and Molecular Therapy,University of Florida

Chapters

  • 00:05Title
  • 01:33Packaging of Recombinant Adeno-associated Virus R=Serotype 3 (rAAV3) Vectors
  • 08:29Determination of rAAV3 Vector Titers
  • 15:11rAAV3 Vector-mediated Transduction and Transgene Expression in Human Liver Cancer Cells
  • 16:55A Representative Quantitative DNA Slot-blot and Representative Transduction of Hepatoblastoma Cells with rAAV3
  • 18:32Conclusion

Summary

Automatic Translation

Automatic Translation

In this article, we describe the identification of the adeno-associated virus serotype 3 (AAV3) as the most efficient vector for targeting human liver cancer cells.

Tags

Recombinant Adeno-associated Virus Serotype 3 VectorsLiver Cancer CellsGene TherapyAAV2AAV SerotypesHuman Liver CancerHepatoblastomaHepatocellular CarcinomaCellular Co-receptorHigh-efficiency TransductionReporter GenePotential Gene Therapy

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