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Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents
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Medicine
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JoVE Journal Medicine
Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents

Orthotopic Xenografting of Human Luciferase-Tagged Malignant Peripheral Nerve Sheath Tumor Cells for in vivo Testing of Candidate Therapeutic Agents

DOI:
10.3791/2558-v

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07:10 min

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March 07, 2011

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, , ,
1Department of Pathology,University of Alabama at Birmingham – UAB, 2Department of Radiology,University of Alabama at Birmingham – UAB, 3Department of Cell Biology and Neurobiology,University of Alabama at Birmingham – UAB

Chapters

  • 00:05Title
  • 01:27Preparation of Non-nude Immunodeficient Mice and MPNST Cell
  • 02:14Grafting Procedure
  • 05:10Bioluminescence as a Measure of Successful Tumor Grafting
  • 06:52Conclusion

Summary

Automatic Translation

Automatic Translation

A method for reliably grafting luciferase-tagged human malignant peripheral nerve sheath tumor cells into the sciatic nerve of immunodeficient mice is described. The use of bioluminescence imaging to demonstrate proper establishment of tumor grafts and criteria for random segregation of animals into study groups are also discussed.

Tags

Orthotopic XenograftingHuman Malignant Peripheral Nerve Sheath Tumor CellsIn Vivo TestingCandidate Therapeutic AgentsAnimal ModelPreclinical TrialsTransgenic MiceXenograft ModelsImmunodeficient MiceTherapeutic Ranges

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