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操纵细胞与细胞间相互作用的硅微芯片
JoVE Journal
Biology
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JoVE Journal
Biology
Silicon Microchips for Manipulating Cell-cell Interaction
Please note that all translations are automatically generated.
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操纵细胞与细胞间相互作用的硅微芯片
DOI:
10.3791/268-v
•
23:21 min
•
August 30, 2007
•
Elliot E Hui
,
Sangeeta N Bhatia
1
Laboratory for Multiscale Regenerative Technologies
,
MIT – Massachusetts Institute of Technology
Chapters
00:06
Introduction
01:17
Device manipulation
03:17
Preparing the parts for cell seeding
05:03
Cell seeding
07:09
Performing the experiment
08:26
Cleaning chips for reuse
13:50
Applying polystyrene coating for reuse
19:35
Discussion
21:43
Applications
22:32
Micro-organization: cell membrane contact vs soluble contact
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了一个微米尺度上的贴壁细胞,细胞与细胞之间的相互作用的动态监管的实验方法。肝细胞和基质细胞之间的连接通讯的操纵表现。开发的平台,使细胞间的相互作用在多种生物学过程,包括开发和发病机制的调查。
Tags
Silicon Microchips
Cell-cell Interaction
Cellular Microenvironment
Malignant Transformation
Neighboring Stroma
Conventional Techniques
Spatial And Dynamic Elements
Micromechanical Reconfigurable Culture (RC)
Micromachined Silicon Substrate
Mechanical Repositioning
Intercellular Communication
Co-cultures Of Hepatocytes And Non-parenchymal Cells
Soluble Signaling
RC System
Handling Device Parts
Actuating Between Gap And Contact Configurations
Preparing Substrates For Culture
Multi-step Cell Seeding Process
Confluent Cell Monolayers
Live Microscopy
Experimental Configurations
Article
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