A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:06Introduction
- 01:17Device manipulation
- 03:17Preparing the parts for cell seeding
- 05:03Cell seeding
- 07:09Performing the experiment
- 08:26Cleaning chips for reuse
- 13:50Applying polystyrene coating for reuse
- 19:35Discussion
- 21:43Applications
- 22:32Micro-organization: cell membrane contact vs soluble contact
Questo articolo descrive un approccio sperimentale per la regolazione dinamica delle interazioni cellula-cellula tra le cellule aderenti su scala micrometrica. Manipolazione della comunicazione intercellulare tra epatociti e cellule stromali è dimostrata. La piattaforma sviluppata consente di indagine di interazioni cellula-cellula in una varietà di processi biologici, tra cui lo sviluppo e la patogenesi.
Tags
Silicon MicrochipsCell-cell InteractionCellular MicroenvironmentMalignant TransformationNeighboring StromaConventional TechniquesSpatial And Dynamic ElementsMicromechanical Reconfigurable Culture (RC)Micromachined Silicon SubstrateMechanical RepositioningIntercellular CommunicationCo-cultures Of Hepatocytes And Non-parenchymal CellsSoluble SignalingRC SystemHandling Device PartsActuating Between Gap And Contact ConfigurationsPreparing Substrates For CultureMulti-step Cell Seeding ProcessConfluent Cell MonolayersLive MicroscopyExperimental Configurations
