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Journal
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Immunology and Infection
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所有流感的定量分析类型使用通用抗体的一种病毒Hemagglutinins和Neuraminidases,在简单的狭缝杂交分析
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
Please note that all translations are automatically generated.
Click here for the English version.
所有流感的定量分析类型使用通用抗体的一种病毒Hemagglutinins和Neuraminidases,在简单的狭缝杂交分析
DOI:
10.3791/2784-v
•
13:12 min
•
April 04, 2011
•
Caroline Gravel*
1
,
Changgui Li*
2
,
Junzhi Wang
,
Anwar M Hashem
3,4
,
Bozena Jaentschke
,
Gary Van Domselaar
,
Runtao He
,
Xuguang Li
3
1
Centre for Vaccine Evaluation, Biologics and Genetic Therapies Directorate, HPFB
,
Health canada
,
2
National Institute for the Control of Pharmaceutical and Biological Products
,
The State Food and Drug Administration, Beijing
,
3
Department of Biochemistry, Microbiology and Immunology
,
University of Ottawa
,
4
Microbiology Department, Faculty of Medicine
,
King Abdulaziz University
,
5
National Microbiology Laboratory
,
Public Health Agency of Canada
Chapters
00:05
Title
02:15
Identification of Hemagglutinin (HA) an Neuraminidase (NA) Conserved Regions
03:04
Slot Blot Reagent Preparation
03:53
Preparation of Influenza Vaccine Samples and Reference Standards
05:20
Assembly of the Bio-Dot SF Microfiltration Apparatus and Sample Blotting
08:08
Immunoblotting of HA and NA Antigens
09:52
Slot Blot Density Analysis
10:40
Universal Antibodies for the Quantification of HA and NA
12:10
Conclusion
Summary
Automatic Translation
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Automatic Translation
一个简单的狭缝杂交方法被开发用于流感病毒的血凝素和使用针对其最保守的序列,通过生物信息学分析确定的普遍抗体神经氨酸酶的量化。这种创新的方法可以提供一个有用的替代所有病毒的血凝素和神经氨酸酶的定量测定。
Tags
Quantitative Analyses
Influenza Type A
Viral Hemagglutinins
Neuraminidases
Universal Antibodies
Simple Slot Blot Assays
Surface Proteins
Viral Life Cycle
Protective Immune Responses
Neutralizing Antibodies
Influenza Vaccine Potency Marker
Single Radial Immunodiffusion (SRID)
Subtype-specific Antisera
New Vaccine Release
NA Antigens
Reagents
Analytical Method
Universally Conserved Regions
Bioinformatics Analyses
Fusion Peptide
Enzymatic Active Site
N-terminus
Article
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