/
/
Analisi quantitativa di tutti influenza di tipo A Hemagglutinins e neuraminidasi virale utilizzando anticorpi Universale di saggi semplice Slot Blot
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Centre for Vaccine Evaluation, Biologics and Genetic Therapies Directorate, HPFB,Health canada, 2National Institute for the Control of Pharmaceutical and Biological Products,The State Food and Drug Administration, Beijing, 3Department of Biochemistry, Microbiology and Immunology,University of Ottawa, 4Microbiology Department, Faculty of Medicine,King Abdulaziz University, 5National Microbiology Laboratory,Public Health Agency of Canada
Chapters
- 00:05Title
- 02:15Identification of Hemagglutinin (HA) an Neuraminidase (NA) Conserved Regions
- 03:04Slot Blot Reagent Preparation
- 03:53Preparation of Influenza Vaccine Samples and Reference Standards
- 05:20Assembly of the Bio-Dot SF Microfiltration Apparatus and Sample Blotting
- 08:08Immunoblotting of HA and NA Antigens
- 09:52Slot Blot Density Analysis
- 10:40Universal Antibodies for the Quantification of HA and NA
- 12:10Conclusion
Un metodo semplice macchia di slot è stato sviluppato per la quantificazione del emoagglutinina e neuraminidasi dell'influenza virale utilizzando anticorpi universali indirizzare le proprie sequenze più conservate identificate attraverso l'analisi bioinformatica. Questo approccio innovativo può fornire una valida alternativa alla determinazione quantitativa di tutti i emoagglutinina e neuraminidasi virale.
Tags
Quantitative AnalysesInfluenza Type AViral HemagglutininsNeuraminidasesUniversal AntibodiesSimple Slot Blot AssaysSurface ProteinsViral Life CycleProtective Immune ResponsesNeutralizing AntibodiesInfluenza Vaccine Potency MarkerSingle Radial Immunodiffusion (SRID)Subtype-specific AntiseraNew Vaccine ReleaseNA AntigensReagentsAnalytical MethodUniversally Conserved RegionsBioinformatics AnalysesFusion PeptideEnzymatic Active SiteN-terminus
