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卡哈尔（ICC）的网络的间质细胞在小鼠体内的可视化
JoVE Journal
Biology
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JoVE Journal Biology
Visualization of the Interstitial Cells of Cajal (ICC) Network in Mice
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卡哈尔（ICC）的网络的间质细胞在小鼠体内的可视化

DOI:
10.3791/2802-v

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09:45 min

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July 27, 2011

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, , , , ,
1Department of Medicine,Memorial Sloan Kettering Cancer Center, 2Human Oncology and Pathogenesis Program,Memorial Sloan Kettering Cancer Center, 3Developmental Biology Program,Memorial Sloan Kettering Cancer Center, 4Howard Hughes,Medical Institute, 5Laboratory of Chromatin Biology and Epigenetics,The Rockefeller University

Chapters

  • 00:05Title
  • 01:35Dissection of the Mouse Gastrointestinal Tract
  • 02:56Whole Mount Sample Preparation and Immunostaining
  • 05:10Cryosection Preparation and Immunostaining
  • 07:44Representative Imaging Results
  • 09:23Conclusion

Summary

Automatic Translation

Automatic Translation

Cajal间的间质细胞（ICC）的胃肠道（GI）的起搏细胞。他们之间的平滑肌细胞和调节胃肠道收缩后神经节神经元纤维形成的复杂的网络。在这里，我们目前的横断面和整个安装小鼠ICC网络的可视化的免疫方法。

Tags

VisualizationInterstitial Cells Of Cajal (ICC)MiceGastrointestinal (GI) TractPacemaker CellsSlow WavesPeristalsisEnteric Nervous SystemMotility DisordersKIT Receptor Tyrosine KinaseKIT ImmunostainingICC NetworkKIT ActivityNeoplastic TransformationGastrointestinal Stromal Tumor (GIST)ETV1 Survival FactorICC/GIST LineageTumorigenesisActivating KIT MutationsVisualization MethodsPreviously Published ProtocolsAnoctamin 1 (ANO1)Membrane MarkerImmunofluorescence

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