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Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5
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Neuroscience
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JoVE Journal Neuroscience
Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5

Screening Assay for Oxidative Stress in a Feline Astrocyte Cell Line, G355-5

DOI:
10.3791/2841-v

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09:53 min

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July 13, 2011

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, , , , , ,
1College of Veterinary Medicine,Western University of Health Sciences, 2Graduate College of Biomedical Sciences,Western University of Health Sciences, 3ReadiSorb,Products

Chapters

  • 00:05Title
  • 01:53Cell Culture of Feline Astrocytes and Treatment with H2O2
  • 05:08Staining for Reactive Oxygen Species
  • 06:45Flow Cytometry
  • 08:18Representative Flow Cytometric Results
  • 09:05Conclusion

Summary

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A screening method to detect oxidative cellular environments is to measure the oxidation of CM-H2DCFDA. Once oxidized within a cell, CM-H2DCFDA changes from non-fluorescent into a fluorescent compound. This change in fluorescence is measured by flow cytometry and indicates the number of cells in an oxidative environment.

Tags

Screening AssayOxidative StressFeline Astrocyte Cell LineVirus-induced Neuronal DamageCentral Nervous System (CNS)AstrocytesReactive Oxygen Species (ROS)CM-H2DCFDAFlow CytometryGreen FluorescenceOxidative StatePathogenesis InvestigationOxygen RadicalRegentCellular ConditionsHydrogen Peroxide

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