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In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening

DOI:

10.3791/58570-v

•

09:49 min

•

November 20, 2018

•

Rosa Crespo, Wouter Koudstaal, Adrian Apetri

¹Janssen Prevention Center,Janssen Pharmaceutical Companies of Johnson and Johnson

Chapters

00:04Title
00:45Continuous Mode ThT Aggregation Assay on a Multi-Mode Microplate Reader
06:43Results: In Vitro Assay to Characterize Tau Protein Aggregation
08:50Conclusion

Summary

Automatic Translation

The tau aggregation assay described in this manuscript mimics the anticipated features of in vivo tau misfolding and aggregation.

Tags

Tau Protein Alzheimer’s Disease Tauopathies Aggregation Assay Drug Screening Nucleation-dependent Polymerization Recombinant Tau Fluorescence SEC-MALS Heparin Thioflavin-T Microplate Reader

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