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Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation
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Neuroscience
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JoVE Journal Neuroscience
Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation

DOI:
10.3791/55974-v

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10:42 min

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July 03, 2017

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,
1Department of Cellular and Physiological Sciences,University of British Columbia

Chapters

  • 00:05Title
  • 00:51Determining Relative Cell-surface Protein Expression in Astrocytes by Biotinylation
  • 04:40Determining Internalization Rate of Cell-surface Proteins in Astrocytes by Biotinylation
  • 08:52Results: AQP4 Expression at the Cell-surface in the Presence of Laminin and AQP4 Internalization in Astrocytes
  • 10:15Conclusion

Summary

Automatic Translation

Automatic Translation

Two biotinylation-based methods, designed for determining the cell-surface expression and endocytic rate of proteins expressed at the plasma membrane, are presented in this report.

Tags

Keywords: Cell-surface BiotinylationPulse-chase BiotinylationAstrocytesAquaporin-4Transmembrane ProteinCell Surface ExpressionProtein InternalizationLamininWater Permeable ChannelPlasma MembraneCell CultureCell LysisProtein PurificationWestern Blot

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