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使用生物素化测定原代星形细胞培养物中蛋白质的细胞表面表达和吞噬率
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Neuroscience
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JoVE Journal Neuroscience
Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation
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使用生物素化测定原代星形细胞培养物中蛋白质的细胞表面表达和吞噬率

DOI:
10.3791/55974-v

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10:42 min

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July 03, 2017

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,
1Department of Cellular and Physiological Sciences,University of British Columbia

Chapters

  • 00:05Title
  • 00:51Determining Relative Cell-surface Protein Expression in Astrocytes by Biotinylation
  • 04:40Determining Internalization Rate of Cell-surface Proteins in Astrocytes by Biotinylation
  • 08:52Results: AQP4 Expression at the Cell-surface in the Presence of Laminin and AQP4 Internalization in Astrocytes
  • 10:15Conclusion

Summary

Automatic Translation

Automatic Translation

本报告提供了两种基于生物素化的方法，设计用于测定在质膜上表达的蛋白质的细胞表面表达和内吞率。

Tags

Cell-surface BiotinylationPulse-chase BiotinylationAstrocytesAquaporin-4Transmembrane ProteinCell Surface ExpressionProtein InternalizationLamininWater Permeable ChannelPlasma MembraneCell CultureCell LysisProtein PurificationWestern Blot

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