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Chapters
- 00:00Title
- 00:18Introduction
- 01:52The SU-8 Master
- 02:51PDMS Replica Molding
- 07:05Preparing the Thin PDMS Valve Layer
- 09:11Plasma Bonding Control and Valve Layers
- 10:37Aligning Control and Fluidic Layers
- 12:02Computer-controlled Opening and Closing of PDMS Microvalves by Vacu
- 15:09An Integrated Microfluidic System for Computer Controlled Perfusion
- 17:32Conclusion
Dimostriamo protocolli per la produzione e l'automazione elastomeriche polidimetilsilossano (PDMS) a base di array microvalvola che non hanno bisogno di energia supplementare per chiudere e dispongono photolithographically definito volumi precisi. Un parallelo subnanoliter-volume del mixer e un sistema integrato di perfusione microfluidica sono presentati.
Tags
Microfluidic ChipsElastomeric Microvalve ArraysPoint-of-care DiagnosticsHigh-throughput Biomedical AssaysFlow ControlFluidic VolumesPolydimethylsiloxane (PDMS)Portable DevicesDeep ChannelsVertical SidewallsPrecise FeaturesThree-layered DevicesFluidic LayerControl LayerThin PDMS MembraneReplica MoldingSU-8 Photoresist MastersOxygen ActivationSolenoid ValvesComputer Software
