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执行自定义的微RNA基因芯片实验
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JoVE Journal Biology
Performing Custom MicroRNA Microarray Experiments
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执行自定义的微RNA基因芯片实验

DOI:
10.3791/3250-v

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07:04 min

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October 28, 2011

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1Department of Pharmacology,University of Minnesota , 2Masonic Cancer Center,University of Minnesota

Chapters

  • 00:05Title
  • 01:10Printing Custom miRNA Microarrays and Sample Preparation
  • 02:23Microarray Hybridization
  • 04:32Post Hybridization Processing
  • 06:03Representative Microarray Results
  • 06:39Conclusion

Summary

Automatic Translation

Automatic Translation

一个简单的程序执行自定义的microRNA微阵列实验描述。这些步骤包括隔离RNA，标签的RNA和参照DNA样本，杂交到芯片，芯片扫描，量化和分析杂交信号。

Tags

Custom MicroRNA MicroarrayMiRNAsRNA MoleculesTarget GenesPost-transcriptionallyBiological ProcessesAltered MiRNA ExpressionHuman DiseasesBiomarkersPrognosisMiRNA Expression ProfilingReal-time PCRDeep SequencingMiRNA Microarray TechniqueHigh-throughputLess ExpensiveMolecular Biology LaboratoryUniversitiesMedical SchoolsHospitalsCustom MiRNA Microarray ExperimentsGlass SlidesRNA IsolationFluorescence Dye

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