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CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
 

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Article DOI: 10.3791/63704-v • 10:40 min • April 25th, 2022
April 25th, 2022 •
1, 1, 1, 1
1Department of Microbiology & Molecular Cell Biology, Leroy T. Canoles Jr. Cancer Research Center, Eastern Virginia Medical School

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Summary
Automatic Translation

This protocol describes a high-throughput clustered regularly interspaced short palindromic repeats (CRISPR) gene editing workflow for microRNA cluster network analysis that allows the rapid generation of a panel of genetically modified cell lines carrying unique miRNA cluster member deletion combinations as large as 35 kb within a single experiment.

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Keywords: CRISPR Gene Editing MicroRNA Cluster Network Analysis High-throughput Cancer Pathways Tumor Growth Drug Resistance Genomic Sequence CRISPR RNA Genotyping Cas9 Doxycycline Western Blot
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