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Neuroscience
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NRCC的膜片钳芯片上细胞的培养和电
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips
Please note that all translations are automatically generated.
Click here for the English version.
NRCC的膜片钳芯片上细胞的培养和电
DOI:
10.3791/3288-v
•
10:36 min
•
February 07, 2012
•
Christophe Py
,
Marzia Martina
,
Robert Monette
,
Tanya Comas
,
Mike W. Denhoff
,
Collin Luk
,
Naweed I. Syed
,
Geoff Mealing
1
Institute for Microstructural Sciences
,
National Research Council of Canada
,
2
Institute for Biological Sciences
,
National Research Council of Canada
,
3
Hotchkiss Brain Institute
,
University of Calgary
Chapters
00:05
Title
02:49
Sterilization, Priming and Testing of Patch-clamp Chips
04:14
Chips Preparation in Cell Biology Lab
07:07
Cell Plating of Snail Neurons
08:45
Electrophysiological Recordings
09:09
Representative Results for the Electrophysiology of Cells on NRCC Patch-clamp Chips
09:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们平面膜片钳芯片如何在加拿大国家研究理事会是消毒催芽,介质加载,与细胞接种,制作,用于电生理记录。
Tags
Culturing
Electrophysiology
Cells
Patch-clamp
Chips
Sensitivity
Ion Channels
Gold Standard
Disease Models
Pharmaceutical Screens
Glass Pipette
Membrane Patch
Electrode
Ion-channel Activity
Whole Cell
Patch-clamp Chips
Suspended Film
Aperture Microfabricated
Automated Systems
High-throughput Screening
Fluidic Delivery
Suction
Cell-to-probe Seals
Whole Cell Mode
Silicon Patch-clamp Chip
Impedance
Orifice Shape
Action Potentials
Snail Neurons
Mammalian Neurons
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