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Chapters
- 00:05Title
- 01:38Preparing the Animal for Sterile Surgery
- 02:53Delivering Tracers in vivo Using Sterile Surgery
- 05:31Neural Circuit Topography of the Cerebellum
- 08:31Conclusion
Vi tilbyr en praktisk guide for å levere sporstoffer<em> In vivo</em> Og bruke spinocerebellar veien som et modellsystem for å demonstrere viktige skritt for å lykkes neuronal kretsanalyse hos mus. Vi beskriver i detalj vår allsidige tracing protokoll som utnytter hvetespirer agglutinin (WGA) konjugert til Alexa fluorophores.
Tags
Neural Circuit TopographyMulti-color Neural CircuitsFunctional Topographic MapsVisualizing Circuit ArchitectureLabeling Topographic ProjectionsCerebellumSpinocerebellar Mossy FibersWheat Germ Agglutinin (WGA)Alexa 555Alexa 488Tracing Neural ProjectionsRetrograde TransportAnterograde TransportTracers PreparationSpinocerebellar Tracing SurgeryThree-dimensional ImagingFunctional Maps OrganizationConnectivity
