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Bioengineering
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荧光检测方法,为微流体液滴平台
JoVE Journal
Bioengineering
Author Produced
This content is Free Access.
JoVE Journal
Bioengineering
Fluorescence detection methods for microfluidic droplet platforms
Please note that all translations are automatically generated.
Click here for the English version.
荧光检测方法,为微流体液滴平台
DOI:
10.3791/3437-v
•
14:16 min
•
December 10, 2011
•
Xavier Casadevall i Solvas
,
Xize Niu
,
Katherine Leeper
,
Soongwon Cho
,
Soo-Ik Chang
,
Joshua B. Edel
,
Andrew J. deMello
1
Department of Chemistry
,
Imperial College London
,
2
Department of Biochemistry, Protein Chip Research Center
,
Chungbuk National University
,
3
Department of Chemistry and Applied Biosciences
,
Institute for Chemical and Bioengineering, ETH Zurich
Chapters
00:05
Title
00:27
Introduction
02:12
Microchip Fabrication
04:07
Microfluidic Setup
05:55
Optical Setup
08:00
Data Acquisition
09:00
Cell Recognition
11:13
Mapping of Mixing Events Using FLIM
13:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
基于液滴微流控平台的高通量实验的有希望的候选人,因为它们能产生廉价的皮升,自我隔离的船只在千赫率。通过整合,快速,灵敏,高分辨率荧光光谱方法,可以有效地提取这些系统内产生的大量信息,利用和利用。
Tags
Fluorescence Detection Methods
Microfluidic Droplet Platforms
System Miniaturization
Nano-to-femto-liter Volumes
Functional Components Integration
Large-scale Multiplexing
Analytical Throughput
Instrumental Footprints Reduction
Droplet-based Microfluidic Systems
High-throughput Experimentation
Water-in-oil Emulsions
Capillary Instabilities
Microdroplets Generation
Isolated Compartments
Sample Cross-talk Elimination
Dispersion Reduction
Minimal Cross-contamination
Accurate Timing Of Analytical Processes
Contact-free Environment
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