Journal
/
Bioengineering
/
Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture

Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture

DOI:
10.3791/3490-v

•

13:37 min

•

February 02, 2012

•

,
1ARTORG Center for Biomedical Engineering,University of Bern

Chapters

  • 00:05Title
  • 02:51Preparing the Bovine Tail for Dissection
  • 04:01Dissection of Intact Bovine Tail Intervertebral Discs
  • 07:02Free-swelling Organ Culture Systems in Beakers / Containers
  • 07:39Organ Culture in Custom-made Specimen Chambers using Glass/PE Containers
  • 08:26Diffusion Experiment to Determine Successful Intervertebral Disc Preparation
  • 09:39Representative Images from Free Swelling and Live/Dead Experiments Performed on IVDs
  • 12:45Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol illustrates a harvesting technique for coccygeal bovine intervertebral discs for organ culture for in vitro organ culture.

Tags

Intact Bovine Tail Intervertebral DiscsOrgan CultureIntervertebral Disc (IVD)SpineNucleus Pulposus (NP)Annulus Fibrosus (AF)Cartilaginous EndplatesDegenerative Intervertebral Disc Disease (DDD)Disc HerniationsDiscogenic PainIn Vitro Organ CultureLive Disc CellsMechano-biological AspectsBovine Coccygeal IVDsHuman Lumbar IVDsCell DensityCell PopulationDimensionsBovine Caudal IVD Harvesting TechniquesCartilaginous EndplatesBony EndplatesAvascular OrgansDiffusionCapillary Buds

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/3490?article_type=v

Alginate Hydrogels for Three-Dimensional Organ Culture of Ovaries and Oviducts
check_url/3490?article_type=v

Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications
check_url/3490?article_type=v

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor
check_url/3490?article_type=v

Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models
check_url/3490?article_type=v

The Multi-organ Chip – A Microfluidic Platform for Long-term Multi-tissue Coculture
check_url/3490?article_type=v

Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy
check_url/3490?article_type=v

An In Vitro Organ Culture Model of the Murine Intervertebral Disc
check_url/3490?article_type=v

Fabrication and Validation of an Organ-on-chip System with Integrated Electrodes to Directly Quantify Transendothelial Electrical Resistance
check_url/3490?article_type=v

Fragility Assessment of Bovine Cortical Bone Using Scratch Tests
check_url/3490?article_type=v

Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips

Read Article