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Imagem intravital da Thymus rato usando 2-Photon Microscopia
JoVE Journal
Immunology and Infection
Author Produced
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JoVE Journal Immunology and Infection
Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy
DOI:

10:08 min

January 07, 2012

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Chapters

  • 00:01Title
  • 00:05Introduction
  • 01:15Animal preparation: Irradiation and bone marrow transfer
  • 03:16Animal preparation: Thymic transplantation
  • 04:58Animal preparation: Surgery to exposure transplanted thymus
  • 06:53Two-photon image acquisition
  • 08:35Results

Summary

Automatic Translation

Nós desenvolvemos novas ferramentas de laboratório e protocolos para aquisição de imagens intravital do timo. Nossa técnica deve ajudar na identificação de "nichos" dentro do timo, onde o desenvolvimento de células T ocorre.

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