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Bioengineering
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在封装的细胞聚集跟踪缺氧信号
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Tracking Hypoxic Signaling within Encapsulated Cell Aggregates
Please note that all translations are automatically generated.
Click here for the English version.
在封装的细胞聚集跟踪缺氧信号
DOI:
10.3791/3521-v
•
09:14 min
•
December 16, 2011
•
Matthew L. Skiles
,
Suchit Sahai
,
James O. Blanchette
1
Biomedical Engineering Program
,
University of South Carolina
,
2
Chemical Engineering Department
,
University of South Carolina
Chapters
00:05
Title
01:47
PEGDM Synthesis
03:12
Culture, Infection, and Aggregation of Cells
04:42
Encapsulation of Cells in PEGDM
06:39
Hypoxia Tracking
07:12
Representative Hydrogels and Fluorescent Hypoxic Signaling
08:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
封装在一个交联的聚乙二醇水凝胶的细胞照片的方法描述。使用荧光标记系统内缺氧的信号封装小鼠胰岛素(MIN6)聚合跟踪。这个系统允许在水凝胶支架和相关的缺氧信号串行检查细胞与细胞表型的变化。
Tags
Tracking
Hypoxic Signaling
Encapsulated Cell Aggregates
Diabetes Mellitus Type 1
Pancreatic Beta-cells
Insulin Production
Hyperglycemia
Exogenous Insulin Injection
Transplantation
Normoglycemia
Immune System
Encapsulation
Biologically-derived Materials
Alginate
Agarose
Inflammation
Fibrotic Overgrowth
Nutrient Transport
Oxygen Transport
Synthetic Poly(ethylene Glycol)
PEG-based Hydrogels
Photo-crosslinking Reaction
UV Degradation
Photoinitiator
Irgacure 2959
Free Radicals
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