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Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
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JoVE Journal Biology
Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

DOI:
10.3791/3583-v

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07:26 min

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January 26, 2012

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, , , , ,
1Department of Energy, Environmental and Chemical Engineering,Washington University, 2Department of Biology,Washington University, 3Department of Energy, Environmental and Chemical Engineering and Department of Biology,Washington University

Chapters

  • 00:05Title
  • 01:16Cell Culture
  • 01:40Amino Acid Extraction
  • 02:46Amino Acid Derivatization and GC-MS Conditions
  • 04:07GC-MS Data Analysis
  • 05:41Representative GC-MS Analysis
  • 07:06Conclusion

Summary

Automatic Translation

Automatic Translation

13C-isotope labeling is a useful technique for determining the cell central metabolism for various types of microorganisms. After cells have been cultured with a specific labeled substrate, GC-MS measurement can reveal functional metabolic pathways based on unique labeling patterns in proteinogenic amino acids.

Tags

Metabolic Pathway ConfirmationMetabolic Pathway Discovery13C LabelingProteinogenic Amino AcidsBiochemistryFunctional Genomics13C-assisted Metabolism AnalysisIsotopic LabelingAtom Transition PathsBiochemical NetworkTranscriptomicsProteomicsIsotopomer-assisted Analysis Of Metabolic PathwaysLabeled SubstratesMinimal MediumCarbon SourceMeasurement NoisesNon-labeled CarbonNutrient SupplementsLabeled Substrate ChoiceReaction StereochemistryIntermediate ProductsSingly Labeled Carbon SubstratesUniformly Labeled SubstratesAmino Acid Labeling PatternsGC-MS

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