Journal
/
/
代谢途径的确认和发现通过 13 C - Proteinogenic氨基酸的标签
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
DOI:

07:26 min

January 26, 2012

, , , , ,

Chapters

  • 00:05Title
  • 01:16Cell Culture
  • 01:40Amino Acid Extraction
  • 02:46Amino Acid Derivatization and GC-MS Conditions
  • 04:07GC-MS Data Analysis
  • 05:41Representative GC-MS Analysis
  • 07:06Conclusion

Summary

Automatic Translation

C - 13同位素标记是一个有用的技术,确定各类微生物细胞中央代谢。后已与一个特定的标记底物培养的细胞,气相色谱 - 质谱测量,可以揭示proteinogenic氨基酸的独特标签模式为基础的功能代谢途径。

Related Videos

Read Article