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Chapters
- 00:05Title
- 01:49Preparing Human Articular Chondrocytes (HAC) for 3D Encapsulation
- 02:51Encapsulating HACs into 3D Beads
- 04:50Culturing Chondrocytes in Synovial Fluid
- 06:05Harvesting HACs in Alginate Beads for Gene Expression or Histological Analysis
- 07:363D Cultures
- 09:31Conclusion
Et 3D-system med dyrkning menneskelige artikulær chondrocytter i høje niveauer af synovialvæsken er beskrevet. Synovialvæske afspejler den mest naturlige mikromiljø for ledbrusken, og kan let udtages og opbevares. Dette system kan derfor bruges til at studere brusk regenerering og til screening af lægemidler til behandling af gigt.
Tags
3D SystemCulturingHuman Articular ChondrocytesSynovial FluidCartilage DestructionOsteoarthritisDisabilityCartilage RegenerationChronic PainImmobilityScaffoldsBalanced MediumIn Vivo TransplantationChondrocyte GrowthMatrix DepositionNative Joint CavitySynovial FluidAvascular Articular CartilageGrowth FactorsCytokinesEnzymesChondrocyte MetabolismHyaluronanLubricinArtificial Media
