交付使用表皮生长因子在人胰腺癌细胞转染基因受体有针对性的明胶基于工程Nanovectors

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

交付使用表皮生长因子在人胰腺癌细胞转染基因受体有针对性的明胶基于工程Nanovectors

DOI:

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08:35 min

January 04, 2012

00:05Title
01:29Preparation of Plasmid DNA Encapsulated EGRF-targeted Gelatin Nanoparticles
05:28Determination of Transfection Efficiency by Fluorescence Microscopy
07:15Quantitative Determination of Therapeutic Efficiency with Plasmid-encapsulated Nanoparticles
08:08Conclusion

系统性基因传递和转染治疗胰腺癌的B型明胶为基础的设计nanovectors系统（氏族）的开发。他们可以通过与表皮生长因子受体（EGFR）的特异性多肽nanparticles表面改性，表皮生长因子受体受体目标和下降低细胞内谷胱甘肽的浓度高，如环境，释放质粒。