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Bioengineering

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JoVE Journal Bioengineering
Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds
 

Electrospinning Growth Factor Releasing Microspheres into Fibrous Scaffolds

Article DOI: 10.3791/51517-v • 09:29 min • August 16th, 2014
August 16th, 2014 •
1, 1
1Biomedical Engineering, Wayne State University

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Summary
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This protocol combines electrospinning and microspheres to develop tissue engineered scaffolds to direct neurons. Nerve growth factor was encapsulated within PLGA microspheres and electrospun into Hyaluronic Acid (HA) fibrous scaffolds. The protein bioactivity was tested by seeding the scaffolds with primary chick Dorsal Root Ganglia and culturing for 4-6 days.

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Keywords: Electrospinning Growth Factor Microspheres Fibrous Scaffold Nerve Cell Growth Mechanical Properties Topographical Cues Adhesion Chemical Signals Hyaluronic Acid Poly(lactic-co-glycolic Acid) PLGA Nerve Growth Factor NGF Protein Viability Extended Release
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