A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:00Water/Oil/Water Double Emulsion Microsphere Production
- 03:52Electrospinning with Microspheres
- 05:28Protein Bioactivity Testing
- 07:41Results: Microspheres within Scaffold Release NGF That Promotes Primary Nerve Cell Growth
- 09:09Conclusion
This protocol combines electrospinning and microspheres to develop tissue engineered scaffolds to direct neurons. Nerve growth factor was encapsulated within PLGA microspheres and electrospun into Hyaluronic Acid (HA) fibrous scaffolds. The protein bioactivity was tested by seeding the scaffolds with primary chick Dorsal Root Ganglia and culturing for 4-6 days.
