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神经元形态的研究方法：<em>体外</em> RNAi技术在胚胎小鼠大脑皮质的电穿孔

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Methods for Study of Neuronal Morphogenesis: Ex vivo RNAi Electroporation in Embryonic Murine Cerebral Cortex

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神经元形态的研究方法：<em>体外</em> RNAi技术在胚胎小鼠大脑皮质的电穿孔

DOI:

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10:12 min

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May 18, 2012

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Sofia B. Lizarraga^2,3, Kathryn R. Coser², Mark Sabbagh², Eric M. Morrow^2,3

¹Department of Molecular, Cellular Biology and Biochemistry,Brown University , ²Institute for Brain Science,Brown University , ³Department of Psychiatry and Human Behavior, Warren Alpert School of Medicine,Brown University

Chapters

00:05Title
01:53Dissection and Electroporation
04:10Embedding and Sectioning Electroporated Cortices
06:53Culturing and Analysis of Organotypic Slices
08:55Images of Electroporated Neurons
09:35Conclusion

Summary

Automatic Translation

我们描述涉及的方法进行快速评估的功能基因在大脑皮层的发展，<em>体外</em>电质粒共表达抑制核糖核酸（RNA干扰）和绿色荧光蛋白在小鼠胚胎皮层。该协议是适合研究神经发育的各个方面，如神经，神经细胞迁移和神经元的形态，包括树突和轴突生长。

Tags

Neuronal Morphogenesis Ex Vivo RNAi Electroporation Embryonic Murine Cerebral Cortex Cerebral Cortex Higher Cognitive Functions Six Layered Structure Inside-first Outside-last Manner Neuronal Migration Regulation Of Neuronal Morphogenesis Primary Cultures Organotypic Slices PSilencer Modified Vector U6 Promoter Double Stranded Hairpin RNA CMV Promoter GFP Protein Neurite Outgrowth Candidate Genes Mosaic Analysis

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