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大鼠门户成纤维细胞的分离<em>原位</em>肝脏灌注

JoVE Journal
Biology

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JoVE Journal Biology

Isolation of Rat Portal Fibroblasts by In situ Liver Perfusion

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大鼠门户成纤维细胞的分离<em>原位</em>肝脏灌注

DOI:

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07:39 min

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June 29, 2012

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Jessica W. Wen, Abby L. Olsen, Maryna Perepelyuk, Rebecca G. Wells

¹Division of Gastroenterology, Hepatology & Nutrition, Department of Pediatrics,The Children’s Hospital of Philadelphia, ²Department of Medicine,University of Pennsylvania

Chapters

00:05Title
01:20Perfusion of the Liver
03:31Preparation of the Biliary Tree and Isolation of the Portal Fibroblast Fraction
06:42Results: Representative Primary Portal Fibroblasts Isolated from Adult Rat Liver
07:21Conclusion

Summary

Automatic Translation

一个从大鼠肝门成纤维细胞的分离技术。肝脏被灌注和消化<em>原位</em>胶原酶，其次是<em>体外</em>消化肝细胞浆和大小选择。这种方法提供了一个门户网站的成纤维细胞的纯人口，而不需要通过文化。

Tags

Isolation Rat Portal Fibroblasts In Situ Liver Perfusion Liver Fibrosis Extracellular Matrix Activated Myofibroblasts Hepatic Stellate Cells Bone Marrow Derived Fibroblasts Biliary Fibrosis Biliary Epithelial Injury Scarring In Vitro Study Cell Population Isolation Technique Outgrowth Technique Liver Perfusion Enzymatic Digestion Size Selection Primary Cells

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