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Chapters
- 00:05Title
- 01:20Perfusion of the Liver
- 03:31Preparation of the Biliary Tree and Isolation of the Portal Fibroblast Fraction
- 06:42Results: Representative Primary Portal Fibroblasts Isolated from Adult Rat Liver
- 07:21Conclusion
En teknik til isolering af portal fibroblaster fra rottelever er beskrevet. Lever er perfunderet og fordøjes<em> In situ</em> Med collagenase, efterfulgt af<em> Ex vivo</em> Fordøjelse af leveren opslæmningen og størrelsen selektion af celler. Denne fremgangsmåde tilvejebringer en ren population af portal fibroblaster uden behov for passage i kultur.
Tags
IsolationRat Portal FibroblastsIn Situ Liver PerfusionLiver FibrosisExtracellular MatrixActivated MyofibroblastsHepatic Stellate CellsBone Marrow Derived FibroblastsBiliary FibrosisBiliary Epithelial InjuryScarringIn Vitro StudyCell PopulationIsolation TechniqueOutgrowth TechniqueLiver PerfusionEnzymatic DigestionSize SelectionPrimary Cells
