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推导人类纤维原细胞的皮肤穿刺活检植文化
JoVE Journal
Medicine
Author Produced
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JoVE Journal Medicine
Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts
DOI:

06:19 min

July 07, 2013

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Chapters

  • 00:01Title
  • 00:11Required Materials
  • 01:09Step 1. Transferring biopsy from tube to cutting dish
  • 02:16Step 2. Dissection of the skin biopsy
  • 03:28Step 3. Transfer biopsy pieces to culture plate
  • 05:19Representative Results
  • 06:38Conclusion

Summary

Automatic Translation

成纤维细胞植体培养协议,从人体皮肤打孔活检是一种技术上的强大和简单的方法来获取皮肤细胞银行约15-20万个细胞在低通道数的4-8周内。

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