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Bioengineering
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在活细胞中的小GTPase活性时空操纵在亚细胞水平上几秒钟的时间刻度
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
Please note that all translations are automatically generated.
Click here for the English version.
在活细胞中的小GTPase活性时空操纵在亚细胞水平上几秒钟的时间刻度
DOI:
10.3791/3794-v
•
10:27 min
•
March 09, 2012
•
Robert DeRose
,
Christopher Pohlmeyer
,
Nobuhiro Umeda
2
,
Tasuku Ueno
2
,
Tetsuo Nagano
,
Scot Kuo
3
,
Takanari Inoue
1
Department of Cell Biology, Center for Cell Dynamics
,
Johns Hopkins University
,
2
Graduate School of Pharmaceutical Sciences
,
University of Tokyo
,
3
Biomedical Engineering
,
Johns Hopkins University
Chapters
00:05
Title
02:17
Transfection of Plasmid DNA
04:02
Preparation of cRb-A Solution
05:22
Microscopy and Light Illumination at the Subcellular Level
07:07
Examining the Effect of RAC Activation on Local Ruffle Formation
09:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
被描述为轻小GTPase活性的时空控制的方法。这种方法是基于FKBP-FRB雷帕霉素诱导的异二聚体和照片囚禁系统。优化的光照射,使时空,时间控制在亚细胞水平的小GTP酶激活。
Tags
Spatio-temporal Manipulation
Small GTPase Activity
Subcellular Level
Seconds
Living Cells
Dynamic Regulation
Rho Family
Guanosine Triphosphatases
Cellular Functions
Visualization
Real Time
Perturbation
Protein Activities
Subcellular Location
Timing
Light-induced Activation
Rapamycin-induced FKBP-FRB Heterodimerization
Photo-caging Method
Rapamycin
Rapid Inducible Activation
Inactivation
Translocation
Plasma Membrane
FRB Anchor
Photo-caging System
Rapamycin Analogs
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