/

/

监视依赖黄素单加氧酶和氧化还原半反应，用停流分光光度法

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

Please note that all translations are automatically generated. Click here for the English version.

监视依赖黄素单加氧酶和氧化还原半反应，用停流分光光度法

DOI:

•

12:08 min

•

March 18, 2012

•

Elvira Romero, Reeder Robinson, Pablo Sobrado

¹Department of Biochemistry,Virginia Polytechnic Institute and State University

Chapters

00:05Title
01:37Anaerobic Buffer
02:24Removing Oxygen from the Stopped-flow System
03:22Key Solutions
04:45Reductive Half-reaction: Monitoring Flavin Reduction
07:31Oxidative Half-reaction: Monitoring Flavin Oxidation
10:10Stopped Flow: Spectral Forms of Af SidA
11:27Conclusion

Summary

Automatic Translation

我们描述了一停流仪器的使用调查还原和氧化半反应<em>曲霉</em>一个铁载体（SIDA），黄素依赖型单加氧酶。然后，我们显示相应的在思达反应的物种的光谱，并计算其形成的速率常数。

Tags

Stopped-flow Spectrophotometry Flavin-dependent Monooxygenase Aspergillus Fumigatus Siderophore A (SidA)Hydroxylation Of Ornithine Biosynthesis Of Hydroxamate Siderophores Reductive Half-reaction Oxidative Half-reaction FAD NADPH C4a-hydroperoxyflavin Intermediate Measurement Of Rates Spectral Forms Stopped-flow Instrument Anaerobic Glove Box Single Mode Double Mixing Settings

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

利用微流控平台的高通量蛋白质表达发生器

在一个容器的支架部署在监测的墙力学

非接触，无标签使用拉曼光谱研究细胞和细胞外基质的监测

质量流式细胞术：协议，每日调整和执行细胞的样品上一个CyTOF的质量流式细胞仪

手术技术组织工程血管移植和后续的植入<em>在体内</em>监控

细胞内胆汁酸动态实时监控使用遗传编码的FRET为基础的胆汁酸传感器

心脏内侧燃光导管,用于监测细胞代谢,使用渗透哺乳动物心脏的透射吸收光谱

人体器官性心脏切片双电压和钙光学映射的临床前心脏电生理评估

使用高速摄像机和图像分析对快速移动微泡区域进行成像和定量

标记细胞外囊泡以监测软骨外植体的迁移和摄取

Read Article