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Chapters
- 00:05Title
- 01:15Culturing Mouse Embryonic Stem Cells (Timing: 3 days)
- 02:34Neural Induction (Timing: 2 days)
- 04:48Motor Neuron Specification (Timing: 5 days)
- 05:24Preparation of Poly-DL-Ornithine/Laminin Coated Coverslips (Timing: 2 days)
- 06:18Axon Elongation (Timing: 2 days)
- 07:47Results: mES Cell Differentiation into Motor Neurons
- 09:12Conclusion
Desenvolvemos um novo protocolo para melhorar a eficiência de diferenciação in vitro de células-tronco embrionárias em neurônios motores. As células-tronco diferenciadas adquirido neurônios motores apresenta como evidenciado pela expressão de marcadores neuronais e neurônio motor utilizando técnicas de imuno-histoquímica.
Tags
Mouse Embryonic Stem CellsMotor NeuronsDifferentiationRetinoic AcidSonic HedgehogDisease MechanismsDrug CompoundsSpinal Muscular AtrophyAmyotrophic Lateral SclerosisProtocolsNeuralization ProcessNogginFibroblast Growth FactorsFGF SignalingNeurogenesisAnterior Neural PatterningPosterior Neural Identity
