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大鼠肝细胞的分离和小学文化
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Isolation and Primary Culture of Rat Hepatic Cells
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大鼠肝细胞的分离和小学文化

DOI:
10.3791/3917-v

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07:06 min

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June 29, 2012

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, , ,
1Department of Pathology and Laboratory Medicine,University of Cincinnati College of Medicine, 2American University in Washington, D.C., 3Department of Internal Medicine,Saint Louis University School of Medicine

Chapters

  • 00:05Title
  • 01:04Rat Perfusion for Liver Isolation
  • 03:26Hepatocyte Cell Isolation
  • 04:42Hepatocyte Culture
  • 06:09Typical Hepatocyte Harvest and 24-hour Culture Results
  • 06:44Conclusion

Summary

Automatic Translation

Automatic Translation

原发性肝细胞提供了一个宝贵的工具来评估生化，分子，在生理有关的实验系统和代谢功能。我们描述了可靠的协议，对大鼠原位肝灌注，一贯产生，最多to1.0×10的可行肝<sup> 8</sup每准备>细胞与细胞之间的88〜96％的存活率。

Tags

IsolationPrimary CultureRat HepatocytesCollagenase PerfusionLiver FunctionDiseasePathophysiologyPharmacologyResearchTechniqueSeglenDissociationPortal VeinNylon FilterCulture PlatesSerum-containing MediumSerum-free MediumSurgical StepsSterile Culture StepsVideo Demonstration

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