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准备从大鼠脾细胞T细胞生长因子

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Biology

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JoVE Journal Biology

Preparing T Cell Growth Factor from Rat Splenocytes

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准备从大鼠脾细胞T细胞生长因子

DOI:

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18:27 min

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October 31, 2007

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Christine Beeton, K. George Chandy

¹Department of Physiology and Biophysics,University of California, Irvine (UCI)

Chapters

00:00Title
00:06Introduction
01:13TCGF Secretion
03:22Harvesting Spleens from Lewis Rats
05:15Preparing Single Cell Suspensions from Rat Spleens
10:23Lysis of Red Blood Cells
12:40Preparing Splenocyte Culture
13:34Elimination of the Conconavalin A
17:53Conclusion

Summary

Automatic Translation

我们描述的T细胞生长因子在体外扩增抗原特异性大鼠T淋巴细胞线的准备。

Tags

T Cell Growth Factor Rat Splenocytes Antigen-specific T Cell Lines Interleukin 2 Th1 Polarization TCGF Preparation Cytokines Large-scale Production Rat Splenocyte Culture Supernatants Concanavalin A Mitogen Cytokine Production Sterile Filtration Storage At -20°C

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