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ラットの脾細胞からT細胞増殖因子の準備
JoVE Journal
Biology
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JoVE Journal Biology
Preparing T Cell Growth Factor from Rat Splenocytes
DOI:

18:27 min

October 31, 2007

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Chapters

  • 00:00Title
  • 00:06Introduction
  • 01:13TCGF Secretion
  • 03:22Harvesting Spleens from Lewis Rats
  • 05:15Preparing Single Cell Suspensions from Rat Spleens
  • 10:23Lysis of Red Blood Cells
  • 12:40Preparing Splenocyte Culture
  • 13:34Elimination of the Conconavalin A
  • 17:53Conclusion

Summary

Automatic Translation

我々は、抗原特異的ラットTリンパ球のラインのin vitroでの拡大のために使用されるT細胞増殖因子の調製を記載。

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