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NADH的荧光成像隔离双心室工作离体兔心

JoVE Journal
Medicine

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JoVE Journal Medicine

NADH Fluorescence Imaging of Isolated Biventricular Working Rabbit Hearts

Please note that all translations are automatically generated. Click here for the English version.

NADH的荧光成像隔离双心室工作离体兔心

DOI:

•

12:07 min

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July 24, 2012

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Huda Asfour, Anastasia M. Wengrowski, Rafael Jaimes III, Luther M. Swift, Matthew W. Kay

¹Electrical and Computer Engineering Department,The George Washington University, ²Pharmacology and Physiology Department,The George Washington University

Chapters

00:05Title
01:25Setting Up for the Study
02:44Heart Excision
03:50Biventricular Cannulation
07:21Signal Acquisition: Pressures, Monophasic Action Potentials, and fNADH
09:08Typical Pressure, Monophasic Action Potentials, and fNADH Data
11:12Conclusion

Summary

Automatic Translation

目标是在生理前，后负荷压力的情况下离体心脏的线粒体氧化还原状态监测。提出了一个双心室工作兔心脏模型。高时空分辨率的NADH荧光成像是用来监测线粒体外膜组织的氧化还原状态。

Tags

NADH Fluorescence Imaging Isolated Perfused Heart Langendorff Technique Cardiac Physiology Cardiac Metabolism LV Working Heart Model Biventricular Model Stroke Volume Cardiac Output Pressure Development Myocardial Injury Ischemia NADH Fluorescence

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