Journal
/
Medicine
/
In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy

In vivo Imaging of Tumor Angiogenesis using Fluorescence Confocal Videomicroscopy

DOI:
10.3791/50347-v

•

05:08 min

•

September 11, 2013

•

, , , , ,
1Université Paris Descartes Sorbonne Paris Cité, INSERM UMR-S970, 2Assistance Publique-Hôpitaux de Paris,Hôpital Européen Georges Pompidou, Service de Radiologie

Chapters

  • 00:05Title
  • 01:34Preparation of the Organ of Interest
  • 02:01Acquisition
  • 02:50Quantitative Analysis of Microcirculation Parameters
  • 03:47Results: Representative in vivo Imaging of Tumor Angiogenesis Using Fluorescence Confocal Videomicroscopy
  • 04:45Conclusion

Summary

Automatic Translation

Automatic Translation

In this paper, we present a method to analyze tumor microvessels in vivo using dynamic contrast-enhanced fluorescence videomicroscopy. Two quantitative parameters were acquired: functional capillary density reflecting the vascularity of the tumor, and index leakage reflecting the leakiness of the endothelial wall.

Tags

In Vivo ImagingTumor AngiogenesisFluorescence Confocal VideomicroscopyFibered Confocal Fluorescence ImagingCapillary MorphologyCapillary FunctionFunctional Capillary DensityMicrovascular DensityCapillary PermeabilityMacromolecular Contrast AgentImage Processing

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50347?article_type=v

In vivo Near Infrared Fluorescence (NIRF) Intravascular Molecular Imaging of Inflammatory Plaque, a Multimodal Approach to Imaging of Atherosclerosis
check_url/50347?article_type=v

In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model
check_url/50347?article_type=v

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound
check_url/50347?article_type=v

A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics
check_url/50347?article_type=v

Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate
check_url/50347?article_type=v

In vivo 19F MRI for Cell Tracking
check_url/50347?article_type=v

In Vivo Optical Imaging of Brain Tumors and Arthritis Using Fluorescent SapC-DOPS Nanovesicles
check_url/50347?article_type=v

Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
check_url/50347?article_type=v

In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma
check_url/50347?article_type=v

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

Read Article