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Trans-vivo Delayed Type Hypersensitivity Assay for Antigen Specific Regulation
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Chapters
- 00:05Title
- 01:54Preparation of Lymphocytes
- 02:43Preparation of Alloantigen
- 03:46Cell Preparation for Injections
- 05:01Pre-measurement, Injection, and Post-measurement
- 07:18Results: Trans-vivo Delayed Type Hypersensitivity Assay
- 10:56Conclusion
We describe a valuable diagnostic assay that could potentially be used to decide the withdrawal of immunosuppression after transplant without elevated risk of graft rejection. The assay uses the principles of Delayed Type Hypersensitivity and provides accurate assessment of both donor specific effector and regulatory immune responses mounted by recipients.
Tags
Trans-vivoDelayed-type Hypersensitivity AssayAntigen-specific RegulationT Cell-dependent Immune ResponseForeign AntigenSensitization PhaseChallenge PhaseAnergyTraditional DTH AssayMicrobial InfectionsTransplant PatientsDirect DTHDiagnostic TechniqueHuman-to-mouseTransfer DTH AssayPeripheral Blood Mononuclear Cells (PBMCs)Specific AntigensNaïve MouseSwelling MeasurementAntigen PresentationMacrophagesDCsInflammatory Cascade
