JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
Donma İnsan ES Hücreler
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Freezing Human ES Cells
Please note that all translations are automatically generated.
Click here for the English version.
Donma İnsan ES Hücreler
DOI:
10.3791/50-v
•
08:00 min
•
October 12, 2006
•
Erin Trish
,
John Dimos
,
Kevin Eggan
1
Department of Molecular and Cell Biology
,
Harvard
Chapters
00:20
Preparation
00:55
Trypsinize cells
05:09
Resuspend cells and store in cryogen tubes
06:29
Add freezing media
06:53
Transfer cells to freezer
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
Burada bizim laboratuvar tonlar insan embriyonik kök hücre hatları donuyor nasıl göstermektedir.
Tags
Freeze
Human ES Cells
Lab
HuES Human Embryonic Stem Cell Lines
Culture
PBS
Residual Media
Trypsin Reaction
Incubate
Rounding
Lifting Off
Pipetting
Conical Tube
HES Cell Media
Spin
Resuspend
Concentration
Freezing Media
Freezing Vials
-80C
Liquid Nitrogen
Long Term Storage
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/50?article_type=v&slug=freezing-human-es-cells
Donmuş Hücreler itibaren Kültür: İnsan Embriyonik kök hücrelerin
check_url/50?article_type=v&slug=freezing-human-es-cells
ES Hücre türetilen nöroepitelyal Hücre Kültürleri
check_url/50?article_type=v&slug=freezing-human-es-cells
İnsan Embriyonik Kök Hücre (ES) yayılması
check_url/50?article_type=v&slug=freezing-human-es-cells
Flaş Dondurma ve E12.5 Fare Beyin Cryosectioning
check_url/50?article_type=v&slug=freezing-human-es-cells
Donma Çözülme ve Ulaştırma Ambalaj Hücreler
check_url/50?article_type=v&slug=freezing-human-es-cells
İnsan Embriyonik Kök Hücreler Donma ve Çözülme
check_url/50?article_type=v&slug=freezing-human-es-cells
Embriyonik Kök Hücreler farklılaşma oligodendrosit Öncüleri
check_url/50?article_type=v&slug=freezing-human-es-cells
Modifiye ES / OP9 Co-Kültür Protokolü Hematopoetik Döl Geliştirilmiş Karakterizasyonu sağlar.
check_url/50?article_type=v&slug=freezing-human-es-cells
Biyogüvenlik Geliştirilmiş Polimer complexed Ecotropic lentivirüs İnsan Hücreleri, İletimi
check_url/50?article_type=v&slug=freezing-human-es-cells
İncelenmesi<em> Drosophila</emIşık Mikroskopi tarafından> Larva Trakeal Terminal Hücreleri
Read Article
